With the exception of the death of Thomas and Martha Wayne, there may be no singular event that impacted the comic book world as the murder of Ben Parker.
Had this tragedy not taken place, it is possible that Peter Parker would have continued using his powers for monetary gain rather than becoming the super hero that would be the flagship character in the Marvel Universe. But like most heroes who go forward to do good, they can never undo the one moment where they completely failed.
Unlike Bruce Wayne, Peter Parker cannot deny that he had the opportunity to stop the murder of his uncle and surrogate father, Ben Parker. Also, Ben Parker leaves us with the one quote that nearly every comic book fan knows by heart.
With great power, comes, great responsibility.